Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.
نویسندگان
چکیده
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 101 49 شماره
صفحات -
تاریخ انتشار 2004